IL-12 (IL12A & IL12B Heterodimer) Protein, Human, Recombinant (His Tag)

Cat: GMP-CT011-H08H
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All IL-12 (IL12A & IL12B Heterodimer) Reagents
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All IL-12 (IL12A & IL12B Heterodimer) Reagents
IL-12 (IL12A & IL12B Heterodimer) Protein, Human, Recombinant (His Tag) Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 10 EU per mg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Arg 23 & Ile 23
Molecule Mass
The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL-12 (IL12A & IL12B Heterodimer) Protein, Human, Recombinant (His Tag) in SDS-PAGE
Human IL12/IL12A & IL12B Protein 15483
IL-12 (IL12A & IL12B Heterodimer) Protein, Human, Recombinant (His Tag) Alternative Names
CLMF Protein, Human;CLMF2 Protein, Human;IL-12A Protein, Human;IL-12B Protein, Human;IL12 Protein, Human;IL12 p70 Protein, Human;IMD28 Protein, Human;IMD29 Protein, Human;NFSK Protein, Human;NKSF Protein, Human;NKSF1 Protein, Human;NKSF2 Protein, Human;P35 Protein, Human
IL-12 (IL12A & IL12B Heterodimer) Background Information
Full Name
interleukin 12A
info info
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