|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10349-ACG|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10349-ACR|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10349-CF|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10349-CH|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10349-CM|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10349-CY|
|人 IFNA10 基因全長cDNA ORF(克隆載體)||HG10349-M|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10349-M-F|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10349-NF|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10349-NH|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10349-NM|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10349-NY|
|人 IFNA10 基因全長cDNA ORF克隆 (表達載體)||HG10349-UT|
Interferon alpha-10 (IFNA10) is a member of the interferon family. Interferons belong to the group of the regulatory glycoproteins, of low molecular mass. They are the products of infected cell-genome, but not virus, as a consequence of the cause answer by different inductors. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs have other functions: they activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they increase the ability of uninfected host cells to resist new infection by virus. Certain host symptoms, such as aching muscles and fever, are related to the production of IFNs during infection. Human IFN are divided on the sequence of amino-acids into three groups: Alpha, Beta and Gamma interferons.