( We provide with IFITM3 qPCR primers for gene expression analysis, HP102793 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG14141-ACG|
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG14141-ACR|
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG14141-CF|
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG14141-CH|
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG14141-CM|
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG14141-CY|
|人 IFITM3 基因全長cDNA ORF(克隆載體)||HG14141-G|
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG14141-NF|
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG14141-NH|
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG14141-NM|
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG14141-NY|
|人 IFITM3 基因全長cDNA ORF克隆 (表達載體)||HG14141-UT|
Interferon-induced transmembrane protein 3 (IFITM3) belongs to the CD225 family. To replicate, viruses must gain access to the host cell's resources. Interferon (IFN) regulates the actions of a large complement of interferon effector genes (IEGs) that prevent viral replication. The interferon inducible transmembrane protein family members, IFITM1, 2 and 3, are IEGs required for inhibition of influenza A virus, dengue virus, and West Nile virus replication in vitro. IFITM3 is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. It is both necessary and sufficient for preventing the emergence of viral genomes from the endosomal pathway. Viral pseudoparticles were inhibited from transferring their contents into the host cell cytosol by IFN, and IFITM3 was required and sufficient for this action. IFITM3 overexpression is sufficient for this phenotype. Moreover, IFITM3 partially resides in late endosomal and lysosomal structures, placing it in the path of invading viruses.