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人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤

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Human IDS cDNA Clone產品資訊
註冊碼:NM_000202.5
cDNA基因長度:1653bp
cDNA基因描述:Full length Clone DNA of Homo sapiens iduronate 2-sulfatase, transcript variant 1 with C terminal Flag tag.
基因別名:MPS2, SIDS
分子種屬:Human
載體:pCMV3-C-FLAG
Plasmid:pCMV3-IDS-Flag
限制性酶切位點:KpnI + XbaI (6kb + 1.69kb)
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human IDS Gene Plasmid Map
Human IDS / MPS2 transcript variant 1 natural ORF mammalian expression plasmid, C-Flag tag
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤 on other vectors
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10337-ACGNT$8875
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10337-ACRNT$8875
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10337-CFNT$7788
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10337-CHNT$7788
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10337-CMNT$7788
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10337-CYNT$7788
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF(克隆載體)HG10337-MNT$2717
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10337-NFNT$7788
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10337-NHNT$7788
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10337-NMNT$7788
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10337-NYNT$7788
人 Iduronate 2-Sulfatase / IDS transcript variant 1 基因全長cDNA ORF克隆 (表達載體)HG10337-UTNT$7788
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研究背景

Iduronate 2-Sulfatase, also known as IDS, is a member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O- and N-sulfate esters from a variety of substrates. The human Iduronate 2-Sulfatase/IDS consists of a signal peptide, a pro peptide and a mature chain that may be further processed into two chains. Among the identified 18 human sulfatases, Iduronate 2-Sulfatase/IDS is required for the lysosomal degradation of the glycosaminoglycans (GAG), heparan sulfate and dermatan sulfate. Multiple mutations in this X-chromosome localized gene result in Iduronate 2-Sulfatase/IDS enzymatic deficiency, and lead to the sex-linked Mucopolysaccharidosis Type II (MPS II ), also known as Hunter Syndrome characterized by the lysosomal accumulation of the GAG and their excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe due to the level of Iduronate 2-Sulfatase/IDS enzyme. Retroviral-mediated Iduronate 2-Sulfatase/IDS gene transfer into lymphoid cells would be a promising gene therapeutic strategy.

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貨號: HG10337-CF
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  • Human IDS / MPS2 transcript variant 1 natural ORF mammalian expression plasmid, C-Flag tag
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