|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10334-ACG|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10334-ACR|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10334-CF|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10334-CH|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10334-CM|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10334-CY|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF(克隆載體)||HG10334-G|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10334-NF|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10334-NH|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10334-NM|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10334-NY|
|人 HVEM/TNFRSF14/CD270 基因全長cDNA ORF克隆 (表達載體)||HG10334-UT|
Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. It is expressed on many immune cells, including T and B cells, NK cells, monocytes, and neutrophils. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA). HVEM provides a stimulatory signal following engagement with LIGHT (TNFSF14) on T cells. In contrast, it can also provide an inhibitory signal to T cells when it binds the B and T lymphocyte attenuator (BTLA), a ligand member of the Immunoglobulin (Ig) superfamily. Thus, HVEM may be viewed as a molecular switch, capable of facilitating both stimulatory and inhibitory cosignaling in T cells. Substantial evidence from both human disease and from experimental mouse models has indicated that dysregulation of the LIGHT-HVEM-BTLA cosignaling pathway can cause inflammation in the lung and in mucosal tissues.