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人 HEXA 基因全長cDNA ORF克隆 (表達載體), C-His 標籤

  • Cynomolgus CD19/B4/CVID3 Gene Plasmid Map 5620
產品資料評論相關產品實驗方法
 HEXA cDNA Clone產品資訊
註冊碼:NM_000520
cDNA基因長度:1635 bp
cDNA基因描述:Full length Clone DNA of Homo sapiens hexosaminidase A (alpha polypeptide) with C terminal His tag.
基因別名:HEXA
分子種屬:Human
載體:pCMV3-C-His
Plasmid:pCMV3-HEXA-His
限制性酶切位點:HindIII + XbaI(6kb+1.64kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1518A/G not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with HEXA qPCR primers for gene expression analysis, HP101571 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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參考資料
  • Norflus,F. et al., 1996, DNA Cell Biol. 15 (2):89-97.
  • Kaufman M., et al., 1997, Hum. Mutat. 10:295-300.
  • Tanaka A., et al., 2003, J. Hum. Genet. 48:571-574.
  • Chen R., et al., 2009, J. Proteome Res. 8:651-661.
  • Burkard T.R., et al., 2011, BMC Syst. Biol. 5:17-17.
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    貨號: HG12037-CH
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