A33 qPCR Primer Pairs, Human

Cat: HP101158
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A33 qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
A33 qPCR Primer Pairs, Human Alternative Names
A33 qPCR Primer Pairs, Human
A33 Background Information

Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I  membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
glycoprotein A33 (transmembrane)
References
  • Heath J.K., et al., 1997, Proc. Natl. Acad. Sci. USA. 94:469-74.
  • Ritter G., et al., 1997, Biochem. Biophys. Res. Commun. 236:682-6.
  • Frey,D. et al., 2008, Cancer Biother Radiopharm  23 (1):65-73.
  • Rageul,J. et al., 2009, Int J Cancer 125 (12):2802-9. 
  • A33 qPCR Primer Pairs, Mouse, Related Products

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    A33 cDNA ORF Clone in Cloning Vector, Mouse MG50559-M
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    A33 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    A33 cDNA ORF Clone in Cloning Vector, Human HG11277-M
    A33 cDNA ORF Clone, Human, untagged HG11277-UT
    A33 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11277-ACGLN
    A33 cDNA ORF Clone, Human, C-GFPSpark® tag HG11277-ACG
    A33 cDNA ORF Clone, Human, C-OFPSpark® tag HG11277-ACR
    A33 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11277-CF
    A33 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11277-NF
    A33 cDNA ORF Clone, Human, C-His tag HG11277-CH
    A33 cDNA ORF Clone, Human, N-His tag HG11277-NH
    A33 cDNA ORF Clone, Human, C-HA tag HG11277-CY
    A33 cDNA ORF Clone, Human, N-HA tag HG11277-NY
    A33 cDNA ORF Clone, Human, C-Myc tag HG11277-CM
    A33 cDNA ORF Clone, Human, N-Myc tag HG11277-NM
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