GAPDH qPCR Primer Pairs, Human

Cat: HP100003
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GAPDH qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
PCR Size:
108bp
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
GAPDH qPCR Primer Pairs, Human Validated Images
Human GAPDH qPCR 4881
GAPDH dissolution curves
Human GAPDH qPCR 4882
GAPDH amplification curves
GAPDH qPCR Primer Pairs, Human Alternative Names
G3PD qPCR Primer Pairs, Human;GAPD qPCR Primer Pairs, Human;HEL-S-162eP qPCR Primer Pairs, Human
GAPDH Background Information

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Full Name
glyceraldehyde-3-phosphate dehydrogenase
References
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
  • GAPDH qPCR Primer Pairs, Canine, Related Products

    Product Name Catalog#(PDF)
    GAPDH cDNA ORF Clone in Cloning Vector, Canine DG70183-U
    GAPDH cDNA ORF Clone, Canine, untagged DG70183-UT
    GAPDH Lentiviral cDNA ORF Clone, Canine, C-GFPSpark® tag DG70183-ACGLN
    GAPDH cDNA ORF Clone, Canine, C-GFPSpark® tag DG70183-ACG
    GAPDH cDNA ORF Clone, Canine, N-GFPSpark® tag DG70183-ANG
    GAPDH cDNA ORF Clone, Canine, C-OFPSpark® tag DG70183-ACR
    GAPDH cDNA ORF Clone, Canine, N-OFPSpark® tag DG70183-ANR
    GAPDH cDNA ORF Clone, Canine, C-DDK (Flag®) tag DG70183-CF
    GAPDH cDNA ORF Clone, Canine, N-DDK (Flag®) tag DG70183-NF
    GAPDH cDNA ORF Clone, Canine, C-His tag DG70183-CH
    GAPDH cDNA ORF Clone, Canine, N-His tag DG70183-NH
    GAPDH cDNA ORF Clone, Canine, C-HA tag DG70183-CY
    GAPDH cDNA ORF Clone, Canine, N-HA tag DG70183-NY
    GAPDH cDNA ORF Clone, Canine, C-Myc tag DG70183-CM
    GAPDH cDNA ORF Clone, Canine, N-Myc tag DG70183-NM

    GAPDH qPCR Primer Pairs, Cynomolgus, Related Products

    Product Name Catalog#(PDF)
    GAPDH cDNA ORF Clone in Cloning Vector, Cynomolgus CG90448-G
    GAPDH cDNA ORF Clone, Cynomolgus, untagged CG90448-UT
    GAPDH Lentiviral cDNA ORF Clone, Cynomolgus, C-GFPSpark® tag CG90448-ACGLN
    GAPDH cDNA ORF Clone, Cynomolgus, C-GFPSpark® tag CG90448-ACG
    GAPDH cDNA ORF Clone, Cynomolgus, N-GFPSpark® tag CG90448-ANG
    GAPDH cDNA ORF Clone, Cynomolgus, C-OFPSpark® tag CG90448-ACR
    GAPDH cDNA ORF Clone, Cynomolgus, N-OFPSpark® tag CG90448-ANR
    GAPDH cDNA ORF Clone, Cynomolgus, C-DDK (Flag®) tag CG90448-CF
    GAPDH cDNA ORF Clone, Cynomolgus, N-DDK (Flag®) tag CG90448-NF
    GAPDH cDNA ORF Clone, Cynomolgus, C-His tag CG90448-CH
    GAPDH cDNA ORF Clone, Cynomolgus, N-His tag CG90448-NH
    GAPDH cDNA ORF Clone, Cynomolgus, C-HA tag CG90448-CY
    GAPDH cDNA ORF Clone, Cynomolgus, N-HA tag CG90448-NY
    GAPDH cDNA ORF Clone, Cynomolgus, C-Myc tag CG90448-CM
    GAPDH cDNA ORF Clone, Cynomolgus, N-Myc tag CG90448-NM

    GAPDH qPCR Primer Pairs, Rabbit, Related Products

    Product Name Catalog#(PDF)
    GAPDH cDNA ORF Clone in Cloning Vector, Rabbit TG65041-G
    GAPDH cDNA ORF Clone, Rabbit, untagged TG65041-UT
    GAPDH Lentiviral cDNA ORF Clone, Rabbit, C-GFPSpark® tag TG65041-ACGLN
    GAPDH cDNA ORF Clone, Rabbit, C-GFPSpark® tag TG65041-ACG
    GAPDH cDNA ORF Clone, Rabbit, N-GFPSpark® tag TG65041-ANG
    GAPDH cDNA ORF Clone, Rabbit, C-OFPSpark® tag TG65041-ACR
    GAPDH cDNA ORF Clone, Rabbit, N-OFPSpark® tag TG65041-ANR
    GAPDH cDNA ORF Clone, Rabbit, C-DDK (Flag®) tag TG65041-CF
    GAPDH cDNA ORF Clone, Rabbit, N-DDK (Flag®) tag TG65041-NF
    GAPDH cDNA ORF Clone, Rabbit, C-His tag TG65041-CH
    GAPDH cDNA ORF Clone, Rabbit, N-His tag TG65041-NH
    GAPDH cDNA ORF Clone, Rabbit, C-HA tag TG65041-CY
    GAPDH cDNA ORF Clone, Rabbit, N-HA tag TG65041-NY
    GAPDH cDNA ORF Clone, Rabbit, C-Myc tag TG65041-CM
    GAPDH cDNA ORF Clone, Rabbit, N-Myc tag TG65041-NM

    GAPDH qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    GAPDH cDNA ORF Clone in Cloning Vector, Rat RG80680-U
    GAPDH cDNA ORF Clone, Rat, untagged RG80680-UT
    GAPDH Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80680-ACGLN
    GAPDH cDNA ORF Clone, Rat, C-GFPSpark® tag RG80680-ACG
    GAPDH cDNA ORF Clone, Rat, N-GFPSpark® tag RG80680-ANG
    GAPDH cDNA ORF Clone, Rat, C-OFPSpark® tag RG80680-ACR
    GAPDH cDNA ORF Clone, Rat, N-OFPSpark® tag RG80680-ANR
    GAPDH cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80680-CF
    GAPDH cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80680-NF
    GAPDH cDNA ORF Clone, Rat, C-His tag RG80680-CH
    GAPDH cDNA ORF Clone, Rat, N-His tag RG80680-NH
    GAPDH cDNA ORF Clone, Rat, C-HA tag RG80680-CY
    GAPDH cDNA ORF Clone, Rat, N-HA tag RG80680-NY
    GAPDH cDNA ORF Clone, Rat, C-Myc tag RG80680-CM
    GAPDH cDNA ORF Clone, Rat, N-Myc tag RG80680-NM

    GAPDH qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    GAPDH cDNA ORF Clone in Cloning Vector, Mouse MG50545-M
    GAPDH cDNA ORF Clone in Cloning Vector, Mouse MG51221-U
    GAPDH cDNA ORF Clone, Mouse, untagged MG51221-UT
    GAPDH cDNA ORF Clone, Mouse, untagged MG50545-UT
    GAPDH cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50545-ACG
    GAPDH Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51221-ACGLN
    GAPDH Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50545-ACGLN
    GAPDH cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51221-ACG
    GAPDH cDNA ORF Clone, Mouse, N-GFPSpark® tag MG50545-ANG
    GAPDH cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51221-ANG
    GAPDH cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50545-ACR
    GAPDH cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51221-ACR
    GAPDH cDNA ORF Clone, Mouse, N-OFPSpark® tag MG50545-ANR
    GAPDH cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51221-ANR
    GAPDH cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51221-CF
    GAPDH cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50545-CF
    GAPDH cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50545-NF
    GAPDH cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51221-NF
    GAPDH cDNA ORF Clone, Mouse, C-His tag MG51221-CH
    GAPDH cDNA ORF Clone, Mouse, C-His tag MG50545-CH
    GAPDH cDNA ORF Clone, Mouse, N-His tag MG51221-NH
    GAPDH cDNA ORF Clone, Mouse, N-His tag MG50545-NH
    GAPDH cDNA ORF Clone, Mouse, C-HA tag MG51221-CY
    GAPDH cDNA ORF Clone, Mouse, C-HA tag MG50545-CY
    GAPDH cDNA ORF Clone, Mouse, N-HA tag MG51221-NY
    GAPDH cDNA ORF Clone, Mouse, N-HA tag MG50545-NY
    GAPDH cDNA ORF Clone, Mouse, C-Myc tag MG51221-CM
    GAPDH cDNA ORF Clone, Mouse, C-Myc tag MG50545-CM
    GAPDH cDNA ORF Clone, Mouse, N-Myc tag MG51221-NM
    GAPDH cDNA ORF Clone, Mouse, N-Myc tag MG50545-NM

    GAPDH qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    GAPDH cDNA ORF Clone in Cloning Vector, Human HG10094-G
    GAPDH cDNA ORF Clone, Human, untagged HG10094-UT
    GAPDH cDNA ORF Clone, Human, C-GFPSpark® tag HG10094-ACG
    GAPDH Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10094-ACGLN
    GAPDH cDNA ORF Clone, Human, N-GFPSpark® tag HG10094-ANG
    GAPDH cDNA ORF Clone, Human, C-OFPSpark® tag HG10094-ACR
    GAPDH cDNA ORF Clone, Human, N-OFPSpark® tag HG10094-ANR
    GAPDH cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10094-CF
    GAPDH cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10094-NF
    GAPDH cDNA ORF Clone, Human, C-His tag HG10094-CH
    GAPDH cDNA ORF Clone, Human, N-His tag HG10094-NH
    GAPDH cDNA ORF Clone, Human, C-HA tag HG10094-CY
    GAPDH cDNA ORF Clone, Human, N-HA tag HG10094-NY
    GAPDH cDNA ORF Clone, Human, C-Myc tag HG10094-CM
    GAPDH cDNA ORF Clone, Human, N-Myc tag HG10094-NM
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