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人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤

    產品資料評論相關產品實驗方法
     FUOM cDNA Clone產品資訊
    註冊碼:NM_001098483.1
    cDNA基因長度:465bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens fucose mutarotase with N terminal Flag tag.
    基因別名:FUCU, FucM, C10orf125
    分子種屬:Human
    載體:pCMV3-N-FLAG
    Plasmid:
    限制性酶切位點:
    Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    序列資訊:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with FUOM qPCR primers for gene expression analysis, HP102631 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤 on other vectors
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG13974-ACGNT$8150
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG13974-ACRNT$8150
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG13974-ANGNT$8150
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG13974-ANRNT$8150
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG13974-CFNT$7060
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG13974-CHNT$7060
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG13974-CMNT$7060
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG13974-CYNT$7060
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF(克隆載體)HG13974-GNT$2720
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG13974-NFNT$7060
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG13974-NHNT$7060
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG13974-NMNT$7060
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG13974-NYNT$7060
    人 FUOM / fucose mutarotase / FucM 基因全長cDNA ORF克隆 (表達載體)HG13974-UTNT$7060
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    Product nameProduct name
    研究背景

    FUOM, also known as fucose mutarotase and FucM, belongs to the RbsD / FucU family. FUOM is involved in the interconversion between alpha- and beta-L-fucoses. L-Fucose has two isforms: alpha-L-fucose (29.5%) and beta-L-fucose (70.5%). The beta-form is metabolized through the salvage pathway. GDP-L-fucose formed either by the de novo or salvage pathways is transported into the endoplasmic reticulum, where it serves as a substrate for N- and O-glycosylations by fucosyltransferases. Fucosylated structures expressed on cell surfaces or secreted in biological fluids are believed to play a critical role in cell-cell adhesion and recognition processes. FUOM mainly exists as homodimer, but also functions as homotetramer, homooctamer, and homodecamer. FUOM's homodimeric form seems catalytically inactive.

    參考資料
  • Deloukas P. et al., 2004, Nature. 429: 375-81.
  • Ota T. et al., 2004, Nat Genet. 36: 40-5.
  • Dongkyu Park. et al., 2007, Glycobiology. 17 (9): 955-62.
  • Size / Price
    貨號: HG13974-NF
    目錄價: 
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