|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10573-ACG|
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10573-ACR|
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10573-CF|
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10573-CH|
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10573-CM|
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10573-CY|
|人 FGF10 / KGF2 基因全長cDNA ORF(克隆載體)||HG10573-M|
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10573-NF|
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10573-NH|
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10573-NM|
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10573-NY|
|人 FGF10 / KGF2 基因全長cDNA ORF克隆 (表達載體)||HG10573-UT|
Fibroblast growth factor 10 (FGF10) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF10 is required for normal branching morphogenesis. It may play a role in wound healing. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.