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人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), C-His 標籤

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Human FAP cDNA Clone產品資訊
註冊碼:NM_004460.2
cDNA基因長度:2283bp
cDNA基因描述:Full length Clone DNA of Homo sapiens fibroblast activation protein, alpha with C terminal His tag.
基因別名:FAPA, DPPIV, DKFZp686G13158, FAP
分子種屬:Human
載體:pCMV3-C-His
Plasmid:
限制性酶切位點:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), C-His 標籤 on other vectors
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10464-ACGNT$8880
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10464-ACRNT$8880
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10464-CFNT$7790
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10464-CHNT$7790
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10464-CMNT$7790
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10464-CYNT$7790
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF(克隆載體)HG10464-MNT$2720
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10464-M-HNT$7790
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10464-NFNT$7790
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10464-NHNT$7790
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10464-NMNT$7790
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10464-NYNT$7790
人 Fibroblast Activation 蛋白 alpha/FAP 基因全長cDNA ORF克隆 (表達載體)HG10464-UTNT$7790
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研究背景

Seprase, also known as 170 kDa melanoma membrane-bound gelatinase , Fibroblast activation protein alpha, Integral membrane serine protease and FAP, is a single-pass type II membrane protein which belongs to the peptidase S9B family. Seprase / FAP is found in cell surface lamellipodia, invadopodia and on shed vesicles. Seprase / FAP appears to act as a proteolytically active 170-kDa dimer, consisting of two 97-kDa subunits. It is a member of the group type II integral serine proteases, which includes dipeptidyl peptidase IV ( DPPIV / CD26 ) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. Seprase / FAP colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Seprase / FAP colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. DPP4 and seprase exhibit multiple functions due to their abilities to form complexes with each other and to interact with other membrane-associated molecules. In association with DPP4, Seprase / FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. Seprase / FAP has a dual function in tumour progression. The proteolytic activity of Seprase has been shown to promote cell invasiveness towards the ECM and also to support tumour growth and proliferation. Seprase / FAP may have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

參考資料
  • Mori,Y. et al., 2004, Oncology. 67 (5-6):411-9.
  • Aertgeerts K., et al., 2005, J. Biol. Chem. 280:19441-19444.
  • Liu T., et al., 2005, J. Proteome Res. 4:2070-2080.
  • Ghersi G., et al., 2006, Cancer Res. 66:4652-4661.
  • O'Brien,P. et al., 2008, Biochim Biophys Acta. 1784 (9):1130-45.
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    貨號: HG10464-CH
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