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人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤

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Human EPOR cDNA Clone產品資訊
註冊碼:NR_033663.1
cDNA基因長度:1008bp
cDNA基因描述:Full length Clone DNA of Homo sapiens erythropoietin receptor, transcript variant 2 with C terminal Flag tag.
基因別名:EPO-R, MGC138358, EPOR
分子種屬:Human
載體:pCMV3-C-FLAG
Plasmid:pCMV3-EPOR-Flag
限制性酶切位點:KpnI + XbaI (6kb + 1.06kb)
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human EPOR Gene Plasmid Map
Human EPOR transcript variant 2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged
Human EPOR Gene Expression validated Image
Human EPOR transcript variant 2 ORF mammalian expression plasmid, C-Flag tag
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Caption:
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤 on other vectors
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG13305-ACGNT$8150
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG13305-ACRNT$8150
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG13305-CFNT$7060
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG13305-CHNT$7060
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG13305-CMNT$7060
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG13305-CYNT$7060
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF(克隆載體)HG13305-GNT$2720
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG13305-NFNT$7060
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG13305-NHNT$7060
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG13305-NMNT$7060
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG13305-NYNT$7060
人 EPOR/Erythropoietin Receptor transcript variant 2 基因全長cDNA ORF克隆 (表達載體)HG13305-UTNT$7060
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研究背景

Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â…  single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.

參考資料
  • Divoky V, et al. (2002) Mouse surviving solely on human erythropoietin receptor (EpoR): model of human EpoR-linked disease. Blood 99(10): 3873-4.
  • Carruthers SG. (2009) A truncated erythropoietin receptor EPOR-T is associated with hypertension susceptibility. Clin Pharmacol Ther. 86(2): 134-6.
  • Baltaziak M, et al. (2009) Relationships of P53 and Bak with EPO and EPOR in human colorectal cancer. Anticancer Res. 29(10):4151-6.
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    貨號: HG13305-CF
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    • Human EPOR transcript variant 2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged
    • Human EPOR transcript variant 2 ORF mammalian expression plasmid, C-Flag tag
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