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Human EGF / Epidermal Growth Factor Protein

    產品資料評論相關產品實驗方法
     EGF 蛋白產品資訊
    分子別稱:Epidermal Growth Factor,HOMG4,URG
    蛋白構建:A DNA sequence encoding the mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was expressed and purified with an initial Met at the N-terminus.
    分子種屬:Human
    表達宿主:E. coli
    #N/A:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
    Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
     EGF 蛋白質控檢測
    純度:> 95 % as determined by SDS-PAGE
    內毒素:< 1 EU per mg protein as determined by the LAL method.
    穩定性:Samples are stable for up to twelve months from date of receipt at -70℃
    預測N端:Met
    分子量:The recombinant human EGF consists of 54 amino acids and has a calculated molecular mass of 6.3 kDa.
    緩衝液:Lyophilized from sterile PBS, pH 7.4
    1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
    2. Please contact us for any concerns or special requirements.
     EGF 蛋白使用指導
    儲存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
    #N/A:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
    Other EGF Recombinant Protein Products
    Epidermal Growth Factor/EGF 研究背景

    EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.

     Epidermal Growth Factor/EGF 參考資料
  • Chen JX, et al. (2011) Involvement of c-Src/STAT3 signal in EGF-induced proliferation of rat spermatogonial stem cells. Mol Cell Biochem. 358(1-2):67-73.
  • Guo Y, et al. (2012) Correlations among ERCC1, XPB, UBE2I, EGF, TAL2 and ILF3 revealed by gene signatures of histological subtypes of patients with epithelial ovarian cancer. Oncol Rep. 27(1):286-92.
  • Kim S, et al. (2012) Smad7 acts as a negative regulator of the epidermal growth factor (EGF) signaling pathway in breast cancer cells. Cancer Lett. 314(2):147-54.
  • Chatterton RT Jr, et al. (2010) Breast ductal lavage for assessment of breast cancer biomarkers. Horm Cancer. 1(4):197-204.
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    貨號: GMP-10605-HNAE-100
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    Datasheet & Documentation

    Contact Us
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”