|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG11224-ACG|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG11224-ACR|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤||HG11224-ANG|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤||HG11224-ANR|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG11224-CF|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG11224-CH|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG11224-CM|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG11224-CY|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF(克隆載體)||HG11224-M|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG11224-NF|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG11224-NH|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG11224-NM|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG11224-NY|
|人 DNMT2 / TRDMT1 基因全長cDNA ORF克隆 (表達載體)||HG11224-UT|
DNMT2, also known as tRNA (cytosine-5-)-methyltransferase, DNA methyltransferase homolog HsaIIP, and TRDMT1, is a member of the DNA methyltransferase family of enzymes. DNMT2 enzymes have been widely conserved during evolution and contain all of the signature motifs of DNA (cytosine-5)-methyltransferases. It contains all 10 sequence motifs that are conserved among m(5)C MTases, including the consensus S:-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide, and its structure is very similar to prokaryotic DNA methyltransferases. DNMT2 has close homologs in plants, insects and Schizosaccharomyces pombe, but no related sequence can be found in the genomes of Saccharomyces cerevisiae or Caenorhabditis elegans. While the biological function of DNMT2 is not yet known, the strong binding to DNA suggests that DNMT2 may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. However, the DNA methyltransferase activity of these proteins is comparatively weak and their biochemical and functional properties remain enigmatic. Recent evidence now shows that Dnmt2 has a novel tRNA methyltransferase activity, raising the possibility that the biological roles of these proteins might be broader than previously thought.