DLL1 ELISA Kit, Human

Cat: KIT11635
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DLL1 ELISA Kit, Human General Information
Product name
DLL1 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant
Sensitivity
7.6 pg/mL
Assay range
15.63-1000 pg/mL
Specificity
Recognizes both recombinant and natural Human DLL1
Interference
Preparations of the following factors at 100 ng/mL in a mid-range recombinant human DLL1 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 208.00 381.00 790.00
SD 6.46 9.44 25.82
CV(%) 3.1% 2.5% 3.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 193.00 375.00 791.00
SD 16.41 13.90 17.15
CV(%) 8.5% 3.7% 2.2%
Recovery
The recovery of Human DLL1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 82 80 -85%
supernatant (n=3) 98 87 -104%
Linearity
Serum
  Recovery of detected
1:2 91%
1:4 107%
1:8 93%
1:16 94%
Supernatant
  Recovery of detected
1:2 89%
1:4 94%
1:8 99%
1:16 118%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This DLL1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human DLL1 protein in Serum,Cell supernatant . It contains recombinant Human DLL1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
DLL1 ELISA Kit, Human Images
Human DLL1 / Delta-like 1 ELISA Kit Standard Curve 105
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human DLL1 / Delta-like 1 ELISA Kit Specificity 232
This assay recognizes both recombinant and natural Human DLL1. The factors listed below were prepared at 100 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
DLL1 ELISA Kit, Human Alternative Names
Delta ELISA Kit, Human;DELTA1 ELISA Kit, Human;DL1 ELISA Kit, Human
DLL1 Background Information

Delta-like protein 1(DLL1), also known as Delta1, a single-pass type I membrane protein which contains one DSL domain and eight EGF-like domains,  acts as a ligand for Notch receptors, and positively regulates T-cell development. DLL1 is proteolytically processed in a similar manner to the Notch receptor, and it has been speculated to participate in bidirectional signaling. The proteolytic processing of DLL1 helps achieve an asymmetry in Notch signaling in initially equivalent myogenic cells and helps sustain the balance between differentiation and self-renewal. Interactions between DLL1 and Notch in trans activate the Notch pathway, whereas DLL1 binding to Notch in cis inhibits Notch signaling. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM10. It had been demonstrated that DLL1 represents a substrate for several other members of the ADAM family. In co-transfected cells, DLL1 is constitutively cleaved by ADAM12, and the N-terminal fragment of DLL1 is released to medium. ADAM12-mediated cleavage of DLL1 is cell density-dependent, takes place in cis orientation, and does not require the presence of the cytoplasmic domain of ADAM12. Full-length DLL1, but not its N- or C-terminal proteolytic fragment, co-immunoprecipitates with ADAM12. By using a Notch reporter construct, we show that DLL1 processing by ADAM12 increases Notch signaling in a cell-autonomous manner. Furthermore, ADAM9 and ADAM17 have the ability to process DLL1. In contrast, ADAM15 does not cleave DLL1, although the two proteins still co-immunoprecipitate with each other. During fetal development, DLL1 is an essential Notch ligand in the vascular endothelium of large arteries to activate Notch1 and maintain arterial identity. DLL1-Notch signaling was required for VEGF receptor expression in fetal arteries.

Full Name
delta-like 1 (Drosophila)
References
  • Dyczynska E, et al. (2007) Proteolytic processing of delta-like 1 by ADAM proteases. J Biol Chem. 282(1): 436-44.
  • Sun D, et al. (2008) The role of Delta-like 1 shedding in muscle cell self-renewal and differentiation. J Cell Sci. 121(Pt 22): 3815-23.
  • Srensen I, et al. (2009) DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries. Blood. 113(22): 5680-8.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    DLL1 Matched ELISA Antibody Pair Set,Human 46.875 pg/ml SEK11635
    info info
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