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人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤

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Human DBH cDNA Clone產品資訊
註冊碼:BC017174
cDNA基因長度:1812bp
cDNA基因描述:Full length Clone DNA of Homo sapiens dopamine beta-hydroxylase (dopamine beta-monooxyge) with N terminal HA tag.
基因別名:DBM, DBH
分子種屬:Human
載體:pCMV3-N-HA
Plasmid:
限制性酶切位點:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤 on other vectors
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG13440-ACGNT$8875
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG13440-ACRNT$8875
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG13440-ANGNT$8875
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG13440-ANRNT$8875
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG13440-CFNT$7788
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG13440-CHNT$7788
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG13440-CMNT$7788
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG13440-CYNT$7788
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF(克隆載體)HG13440-GNT$2717
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG13440-NFNT$7788
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG13440-NHNT$7788
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG13440-NMNT$7788
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG13440-NYNT$7788
人 DBH / Dopamine beta-Hydroxylase 基因全長cDNA ORF克隆 (表達載體)HG13440-UTNT$7788
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研究背景

DBH is a 290 kDa copper-containing oxygenase. It can be detected in noradrenergic nerve terminals of the central and peripheral nervous systems, and is also expressed in chromaffin cells of the adrenal medulla. DBH contains our identical subunits, and its activity requires ascorbate as a cofactor. It functions in in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine the only transmitter synthesized inside vesicles. DBH has been shown to be associated with decision making and addictive behaviors such as alcohol and smoking, attention deficit hyperactivity disorder, and also with neurological diseases such as Schizophrenia and Alzheimer's.

參考資料
  • Rush RA. et al., 1980, Crit Rev Clin Lab Sci. 12 (3): 241-77.
  • Goldstein M. et al., 1964, Life Sci. 3 (7): 763-7.
  • S Friedman. et al., 1966, The Journal of Biological Chemistry. 241 (10): 2256-9.
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    貨號: HG13440-NY
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