Cystatin B ELISA Kit, Human

Cat: KIT10435
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Cystatin B ELISA Kit, Human General Information
Product name
Cystatin B ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
6.9 pg/mL
Assay range
15.63-1000 pg/mL
Specificity
Recognizes both recombinant and natural Human Cystatin B
Interference
Preparations of DPP7 at 10 ng/mL in a mid-range recombinant human CSTB control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.12 0.24 0.53
SD 0.01 0.01 0.03
CV(%) 5.8% 3.2% 6.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.17 0.34 0.68
SD 0.02 0.03 0.06
CV(%) 10.0% 7.8% 9.6%
Recovery
The recovery of Human CSTB spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=4) 114 109-119%
Linearity
Serum
  Recovery of detected
1:2 107%
1:4 98%
1:8 92%
1:16 99%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This Cystatin B ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Cystatin B protein in Serum . It contains recombinant Human Cystatin B, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Cystatin B ELISA Kit, Human Images
Human Cystatin B / CSTB ELISA Kit Standard Curve 66
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human Cystatin B / CSTB ELISA Kit Specificity 236
This assay recognizes both recombinant and natural Human CSTB. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of DPP7 at 10 ng/mL in a mid-range recombinant human CSTB control were assayed for interference. No significant interference was observed.
Cystatin B ELISA Kit, Human Alternative Names
CST6 ELISA Kit, Human;EPM1 ELISA Kit, Human;EPM1A ELISA Kit, Human;PME ELISA Kit, Human;STFB ELISA Kit, Human;ULD ELISA Kit, Human
Cystatin B Background Information

Cystatin-B, also known as CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB and CST6, is a cytoplasm and nucleus protein which belongs to the cystatin family. Cystatin-B / CSTB is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Cystatin-B / CSTB is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Cystatin-B / CSTB is also thought to play a role in protecting against the proteases leaking from lysosomes. Defects in Cystatin-B / CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) which is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).

Full Name
cystatin B (stefin B)
References
  • Ritonja A., et al.,(1985), Amino acid sequence of the intracellular cysteine proteinase inhibitor cystatin B from human liver. Biochem. Biophys. Res. Commun. 131:1187-1192.
  • Pennacchio L.A., et al., (1996), Mutations in the gene encoding cystatin B in progressive myoclonus epilepsy (EPM1).Science 271:1731-1734.
  • Hattori M., et al.,(2000), The DNA sequence of human chromosome 21.Nature 405:311-319.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
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