C2 ELISA Kit, Human

Cat: KIT10154
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C2 ELISA Kit, Human General Information
Product name
C2 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant
Sensitivity
110.77 pg/mL
Assay range
234.38-15000 pg/mL
Specificity
Recognizes both recombinant and natural Human C2
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 2,165.00 4,176.00 7,840.00
SD 114.79 151.29 212.45
CV(%) 5.3% 3.6% 2.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,994.00 4,188.00 8,025.00
SD 195.35 306.31 742.76
CV(%) 9.8% 7.3% 9.3%
Recovery
The recovery of Human C2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 98 88-105%
supernatant (n=3) 102 97 -107%
Linearity
Serum
  Recovery of detected
1:2 92%
1:4 103%
1:8 105%
1:16 104%
Supernatant
  Recovery of detected
1:2 101%
1:4 99%
1:8 97%
1:16 81%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This C2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human C2 protein in Serum,Cell supernatant . It contains recombinant Human C2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
C2 ELISA Kit, Human Images
Human Complement Component C2 ELISA Kit Standard Curve 40
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human Complement Component C2 ELISA Kit Specificity 240
This assay recognizes both recombinant and natural Human CO2. Human C1S and C5a were prepared at 300 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
C2 ELISA Kit, Human Alternative Names
ARMD14 ELISA Kit, Human;CO2 ELISA Kit, Human
C2 Background Information

Complement component C2 is part of the classical complement pathway which plays a major role in innate immunity against infection. C2 is a glycoprotein synthesized in liver hepatocytes and several other cell types in extrahepatic tissues. This pathway is triggered by a multimolecular complex C1, and subsequently the single-chain form of C2 is cleaved into two chains referred to C2a and C2b by activated C1. The second component of complement (C2) is a multi-domain serine protease that provides catalytic activity for the C3 and C5 convertases of the classical and lectin pathways of human complement. C4b and C2 was investigated by surface plasmon resonance. C2a containing a serine protease domain combines with complement component C4b to form the C3 convertase C4b2a which is responsible for C3 activation, and leads to the stimulation of adaptive immune responses via Lectin pathway. C2 bound to C4b is cleaved by classical (C1s) or lectin (MASP2) proteases to produce C4bC2a. C2 has the same serine protease domain as C4bC2a but in an inactive zymogen-like conformation, requiring cofactor-induced conformational change for activity. Deficiency of C2 (C2D) is the most common genetic deficiency of the complement system, and two types of C2D have been recognized in the context of specific MHC haplotypes. C2D in human is reported to increase susceptibility to infection, and is associated with certain autoimmune diseases, such as rheumatological disorders.

Full Name
complement component 2
References
  • Laich A, et al. (2002) Complement C4bC2 complex formation: an investigation by surface plasmon resonance. Biochim Biophys Acta. 1544(1-2): 96-112.
  • Halili MA, et al. (2009) Complement component C2, inhibiting a latent serine protease in the classical pathway of complement activation. Biochemistry. 48(35): 8466-72.
  • Krishnan V, et al. (2009) The structure of C2b, a fragment of complement component C2 produced during C3 convertase formation. Acta Crystallogr D Biol Crystallogr. 65(Pt 3): 266-74.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    C2 Matched ELISA Antibody Pair Set,Human 78.125 pg/ml SEK10154
    info info
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