|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG11228-ACG|
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG11228-ACR|
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG11228-CF|
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG11228-CH|
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG11228-CM|
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG11228-CY|
|人 Carboxypeptidase M Gene cDNA clone plasmid||HG11228-M|
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG11228-NF|
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG11228-NH|
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG11228-NM|
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG11228-NY|
|人 Carboxypeptidase M/CPM 基因全長cDNA ORF克隆 (表達載體)||HG11228-UT|
Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.