CXCL9 ELISA Kit, Human

Cat: KIT10888

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CXCL9 ELISA Kit, Human General Information
Product name
CXCL9 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Detection antibody conjugate
Sample type
7.3 pg/mL
Assay range
15.63-1000 pg/mL
Recognizes both recombinant and natural Human CXCL9
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.14 0.32 0.65
SD 0.01 0.01 0.04
CV(%) 4.8% 4.6% 5.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.14 0.30 0.60
SD 0.01 0.01 0.03
CV(%) 4.4% 4.0% 5.7%
The recovery of Human CXCL9 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 74 70%-82%
  Recovery of detected
1:2 106%
1:4 101%
1:8 81%
1:16 0%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Sample Dilution Buffer Concentrate
7. Color Reagent A
8. Color Reagent B
9. Stop Solution
Product overview
This CXCL9 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL9 protein in Serum . It contains recombinant Human CXCL9, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
This ELISA Kit is shipped at ambient temperature.
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
CXCL9 ELISA Kit, Human Images
Human CXCL9 / MIG ELISA Kit Standard Curve 87
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human CXCL9 / MIG ELISA Kit Specificity 234
This assay recognizes both recombinant and natural human CXCL9. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CXCL9 ELISA Kit, Human Alternative Names
CMK ELISA Kit, Human;crg-10 ELISA Kit, Human;Humig ELISA Kit, Human;MIG ELISA Kit, Human;SCYB9 ELISA Kit, Human
CXCL9 Background Information

Chemokine (C-X-C motif) ligand 9 (CXCL9), also known as Monokine induced by gamma interferon (MIG), is a small cytokine belonging to the CXC chemokine family. The function of this chemokine has not been specifically defined; however, it is thought to be involved in T cell trafficking. CXCL9/MIG functions as one of the three ligands of chemokine receptor CXCR3 which is a G protein-coupled receptor found predominantly on T cells. CXCL9/MIG, together with CXCL10 and CXCL11, may activate CXCR3 by binding to it. CXCL9 serves as a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. It has been observed that tumour endothelial cells secrete high levels of CXCL9 in all, and CXCL10 in most melanoma metastases. Experiment data represent novel mechanisms by which tumour cells in melanoma metastases might use the chemokine-expressing endothelium to leave the tumour and eventually to form additional metastases at distinct sites. Experiment results also improved that CXCL9/MIG plays an important role in CD4+ T lymphocyte recruitment and development of CAV, MOMA-2+ macrophages are the predominant recipient-derived source of CXCL9/MIG, and recipient CD4 lymphocytes are necessary for sustained CXCL9/MIG production and CAV development in this model. Neutralization of the chemokine CXCL9/MIG may have therapeutic potential for the treatment of chronic rejection after heart transplantation.

Full Name
chemokine (C-X-C motif) ligand 9
  • Ruehlmann JM, et al. (2001) MIG (CXCL9) chemokine gene therapy combines with antibody-cytokine fusion protein to suppress growth and dissemination of murine colon carcinoma. Cancer Res. 61(23): 8498-503.
  • Belperio JA, et al. (2003) Role of CXCL9/CXCR3 chemokine biology during pathogenesis of acute lung allograft rejection. J Immunol. 171(9): 4844-52.
  • Colvin RA, et al. (2004) Intracellular domains of CXCR3 that mediate CXCL9, CXCL10, and CXCL11 function. J Biol Chem. 279(29): 30219-27.
  • Valbuena G, et al. (2003) Expression analysis of the T-cell-targeting chemokines CXCL9 and CXCL10 in mice and humans with endothelial infections caused by rickettsiae of the spotted fever group. Am J Pathol. 163(4): 1357-69.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    CXCL9 Matched ELISA Antibody Pair Set,Human 4.6875 pg/ml SEK10888
    info info
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