|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG11647-ACG|
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG11647-ACR|
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG11647-CF|
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG11647-CH|
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG11647-CM|
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG11647-CY|
|人 CTHRC1 基因全長cDNA ORF(克隆載體)||HG11647-M|
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG11647-NF|
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG11647-NH|
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG11647-NM|
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG11647-NY|
|人 CTHRC1 基因全長cDNA ORF克隆 (表達載體)||HG11647-UT|
Collagen triple helix repeat-containing protein 1, also known as Protein NMTC1, and CTHRC1, is a secreted protein that is glycosylated and highly conserved from lower chordates to mammals. CTHRC1 expression was not detectable in normal arteries. However, it is transiently expressed in the arterial wall in response to injury where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration. A short collagen motif with 12 Gly-X-Y repeats appears to be responsible for trimerization of the CTHRC1 protein and this renders the molecule susceptible to cleavage by collagenase. CTHRC1 overexpression caused a dramatic reduction in collagen type I mRNA and protein levels. Currently available data indicate that Cthrc1 expression in vascular cells regulates transforming growth factor beta responsiveness, thereby impacting transforming growth factor beta target genes, including collagens. Additionally, CTHRC1 increases bone mass as a positive regulator of osteoblastic bone formation and offers an anabolic approach for the treatment of osteoporosis.