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人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤

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Human CRIPT cDNA Clone產品資訊
註冊碼:BC006980
cDNA基因長度:306bp
cDNA基因描述:Full length Clone DNA of Homo sapiens cysteine-rich PDZ-binding protein with N terminal Flag tag.
基因別名:HSPC139
分子種屬:Human
載體:pCMV3-N-FLAG
Plasmid:
限制性酶切位點:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤 on other vectors
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG14560-ACGNT$8150
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG14560-ACRNT$8150
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG14560-ANGNT$8150
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG14560-ANRNT$8150
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG14560-CFNT$7060
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG14560-CHNT$7060
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG14560-CMNT$7060
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG14560-CYNT$7060
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF(克隆載體)HG14560-GNT$2720
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG14560-NFNT$7060
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG14560-NHNT$7060
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG14560-NMNT$7060
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG14560-NYNT$7060
人 CRIPT / cysteine-rich PDZ-binding 蛋白 基因全長cDNA ORF克隆 (表達載體)HG14560-UTNT$7060
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研究背景

CRIPT, also known as cysteine-rich PDZ-binding protein, belongs to the CRIPT family. It interacts with TUBB1. CRIPT also interacts strongly with the PDZ3 domain of members of the DLG4 family. It is involved in the cytoskeletal anchoring of DLG4 in excitatory synapses. CRIPT is highly conserved from mammals to plants and binds selectively to the third PDZ domain (PDZ3) of PSD-95 via its C terminus. n heterologous cells, CRIPT causes a redistribution of PSD-95 to microtubules. In brain, CRIPT colocalizes with PSD-95 in the postsynaptic density and can be coimmunoprecipitated with PSD-95 and tubulin. These findings suggest that CRIPT may regulate PSD-95 interaction with a tubulin-based cytoskeleton in excitatory synapses.

參考資料
  • Niethammer M. et al., 1998,Neuron. 20 (4): 693-707.
  • Passafaro M. et al., 2000, Nat Neurosci. 2 (12): 1063-9.
  • Piserchio A. et al., 2002, J Biol Chem. 277 (9): 6967-73.
  • Fukunaga Y. et al., 2005, J Biochem. 138 (2): 177-82.
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    貨號: HG14560-NF
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