|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 CD21 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10811-ACG|
|人 CD21 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10811-ACR|
|人 CD21 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10811-CF|
|人 CD21 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10811-CH|
|人 CD21 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10811-CM|
|人 CD21 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10811-CY|
|人 CD21 基因全長cDNA ORF(克隆載體)||HG10811-M|
|人 CD21 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10811-NF|
|人 CD21 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10811-NH|
|人 CD21 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10811-NM|
|人 CD21 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10811-NY|
|人 CD21 基因全長cDNA ORF克隆 (表達載體)||HG10811-UT|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD21, also known as Complement component (3d / Epstein Barr virus) receptor 2 and CR2, is a member of the CD system and is a protein involved in complement system. CD21 is present on all mature B-cells and some T-cells and follicular dendritic cells. CD21 on mature B-cells form a complex called the B cell receptor complex with two other membrane proteins, CD19 and CD81. CD21 has a function in the complement system through serving as the cellular receper specific for ligands such as C3 and C4 which can be attached to foreign macromolecules in order to remove or uptake them. This results in B-cells having enhanced response to the antigen.