COCH cDNA ORF Clone, Human, N-His tag

Cat: HG11368-NH

Contact us:

215-583-7898
order@sinobiological.com
Distributors

All COCH Reagents

CRO Services

Product recommended


All COCH Reagents

COCH cDNA ORF Clone, Human, N-His tag General Information

Gene

Species
Human
NCBI Ref Seq
RefSeq ORF Size
1653 bp
Description
Full length Clone DNA of Human coagulation factor C homolog, cochlin (Limulus polyphemus) with N terminal His tag.

Plasmid

Promoter
Enhanced CMV promoter
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression

Storage & Shipping

Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

COCH cDNA ORF Clone, Human, N-His tag Alternative Names

COCH-5B2 cDNA ORF Clone, Human;COCH5B2 cDNA ORF Clone, Human;DFNA9 cDNA ORF Clone, Human

COCH Background Information

Cochlin, also known as COCH-5B2 and COCH, is a secreted protein which contains one LCCL domain and two VWFA domains. It is an abundant inner ear protein expressed as multiple isoforms. Its function is also unknown, but it is suspected to be an extracellular matrix component. Cochlin and type II collagen are major constituents of the inner ear extracellular matrix, and Cochlin constitutes 70% of non-collagenous protein in the inner ear, the cochlin isoforms can be classified into three subgroups, p63s, p44s and p40s. The expression of cochlin is highly specific to the inner ear. Eleven missense mutation and one in-frame deletion have been reported in the COCH gene, causing hereditary progressive sensorineural hearing loss and vestibular dysfunction, deafness autosomal dominant type 9 (DFNA9). The co-localization of cochlin and type II collagen in the fibrillar substance in the subepithelial area indicate that cochlin may play a role in the structural homeostasis of the vestibule acting in concert with the fibrillar type II collagen bundles. Defects in COCH may contribute to Meniere disease which is an autosomal dominant disorder characterized by hearing loss associated with episodic vertigo.

Full Name
cochlin
References
  • Ikezono T, et al. (2005) Expression of cochlin in the vestibular organ of rats. ORL J Otorhinolaryngol Relat Spec. 67(5): 252-8.
  • Shindo S, et al. (2008) Spatiotemporal expression of cochlin in the inner ear of rats during postnatal development. Neurosci Lett. 444(2): 148-52.
  • Hosokawa S, et al. (2010) Ultrastructural localization of cochlin in the rat cochlear duct. Audiol Neurootol. 15(4): 247-53.
  • COCH cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    COCH cDNA ORF Clone in Cloning Vector, Mouse MG50943-G
    COCH cDNA ORF Clone, Mouse, untagged MG50943-UT
    COCH Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50943-ACGLN
    COCH cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50943-ACG
    COCH cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50943-ACR
    COCH cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50943-CF
    COCH cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50943-NF
    COCH cDNA ORF Clone, Mouse, C-His tag MG50943-CH
    COCH cDNA ORF Clone, Mouse, N-His tag MG50943-NH
    COCH cDNA ORF Clone, Mouse, C-HA tag MG50943-CY
    COCH cDNA ORF Clone, Mouse, N-HA tag MG50943-NY
    COCH cDNA ORF Clone, Mouse, C-Myc tag MG50943-CM
    COCH cDNA ORF Clone, Mouse, N-Myc tag MG50943-NM
    COCH qPCR Primer Pairs, Mouse MP200912

    COCH cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    COCH cDNA ORF Clone in Cloning Vector, Human HG11368-M
    COCH cDNA ORF Clone, Human, untagged HG11368-UT
    COCH cDNA ORF Clone, Human, C-GFPSpark® tag HG11368-ACG
    COCH Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11368-ACGLN
    COCH cDNA ORF Clone, Human, C-OFPSpark® tag HG11368-ACR
    COCH cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11368-CF
    COCH cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11368-NF
    COCH cDNA ORF Clone, Human, C-His tag HG11368-CH
    COCH cDNA ORF Clone, Human, C-HA tag HG11368-CY
    COCH cDNA ORF Clone, Human, N-HA tag HG11368-NY
    COCH cDNA ORF Clone, Human, C-Myc tag HG11368-CM
    COCH cDNA ORF Clone, Human, N-Myc tag HG11368-NM
    COCH qPCR Primer Pairs, Human HP101250
    info info
    添加购物车成功! 添加购物车失败!