|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10174-ACG|
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10174-ACR|
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10174-CF|
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10174-CH|
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10174-CM|
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10174-CY|
|人 Contactin 3/CNTN3 基因全長cDNA ORF(克隆載體)||HG10174-M|
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10174-NF|
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10174-NH|
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10174-NM|
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10174-NY|
|人 Contactin 3/CNTN3 基因全長cDNA ORF克隆 (表達載體)||HG10174-UT|
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-3, also known as CNTN3 ( BIG-1 in rat and PANG in mouse ), is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. In the brain, Contactin-3 is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.