|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10559-ACG|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10559-ACR|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10559-CF|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10559-CH|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10559-CM|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10559-CY|
|人 DC-SIGNR/CD299 基因全長cDNA ORF(克隆載體)||HG10559-M|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10559-M-F|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體)||HG10559-M-N|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10559-NF|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10559-NH|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10559-NM|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10559-NY|
|人 DC-SIGNR/CD299 基因全長cDNA ORF克隆 (表達載體)||HG10559-UT|
C-type lectin domain family 4, member M, also known as DC-SIGNR and CLEC4M, is a type II integral membrane protein that is 77% amino acid identical to DC-SIGN, an HIV gp120-binding protein. Though the encoded gene located in the same chromosome, DC-SIGN is expressed solely on dendritic cells, while DC-SIGNR is predominantly found in liver sinusoidal endothelial cells and lymph node, as well as placental endothelium. DC-SIGNR exists as a homotetramer, and the tandem repeat domain, also called neck domain, mediates oligermerization. DC-SIGNR is ragarded as a pathogen-recognition receptor involved in peripheral immune surveillance in liver, and probably mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. DC-SIGNR appears to selectively recognize and bind many viral surface glycoproteins containing high mannose N-linked oligosaccharides in a calcium-dependent manner, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S, as well as the cellular adhesion protein ICAM3. DC-SIGNR have been thought to play an important role in establishing HIV infection by enhancing trans-infection of CD4(+)T cells in the regional lymph nodes. It may affect susceptibility to HIV infection by a mechanism that is different in females and males. DC-SIGNR can bind to hepatitis C virus (HCV), and its polymorphism might affect HCV loads supporting the concept that DC-SIGNR contributes to HCV replication efficacy.