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人 CHST11 / C4ST-1 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤

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Human CHST11 cDNA Clone產品資訊
註冊碼:NM_001173982.1
cDNA基因長度:1059bp
cDNA基因描述:Full length Clone DNA of Homo sapiens carbohydrate (chondroitin 4) sulfotransferase 11 with N terminal His tag.
基因別名:C4ST, C4ST1, C4ST-1, HSA269537, CHST11
分子種屬:Human
載體:pCMV3-SP-N-His
Plasmid:
限制性酶切位點:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

人 CHST11 / C4ST-1 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤 on other vectors
Product nameProduct name
研究背景

CHST11, also known as C4ST-1, belongs to the sulfotransferase 2 family. CHST11 localizes to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. A chromosomal translocation involving CHST11 gene and IgH, t(12;14)(q23;q32), has been reported in a patient with B-cell chronic lymphocytic leukemia.

參考資料
  • Hiraoka N. et al., 2000, J Biol Chem. 275 (26): 20188-96.
  • Schmidt HH. et al., 2004, Oncogene. 23 (41): 6991-6.
  • Okuda T. et al., 2001, J Biochem. 128 (5): 763-70.
  • Size / Price
    貨號: HG11396-NH
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