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人 CHST11 / C4ST-1 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤

    產品資料評論相關產品實驗方法
     CHST11 cDNA Clone產品資訊
    註冊碼:NM_001173982.1
    cDNA基因長度:1059bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens carbohydrate (chondroitin 4) sulfotransferase 11 with N terminal His tag.
    基因別名:C4ST, C4ST1, C4ST-1, HSA269537, CHST11
    分子種屬:Human
    載體:pCMV3-SP-N-His
    Plasmid:
    限制性酶切位點:
    Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
    序列資訊:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with CHST11 qPCR primers for gene expression analysis, HP101273 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    His Tag Info

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

    Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

    人 CHST11 / C4ST-1 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤 on other vectors
    Product nameProduct name
    研究背景

    CHST11, also known as C4ST-1, belongs to the sulfotransferase 2 family. CHST11 localizes to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. A chromosomal translocation involving CHST11 gene and IgH, t(12;14)(q23;q32), has been reported in a patient with B-cell chronic lymphocytic leukemia.

    參考資料
  • Hiraoka N. et al., 2000, J Biol Chem. 275 (26): 20188-96.
  • Schmidt HH. et al., 2004, Oncogene. 23 (41): 6991-6.
  • Okuda T. et al., 2001, J Biochem. 128 (5): 763-70.
  • Size / Price
    貨號: HG11396-NH
    目錄價: 
    單價:      (You Save: )

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