( We provide with CHI3L2 qPCR primers for gene expression analysis, HP101796 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG12162-ACG|
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG12162-ACR|
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG12162-CF|
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG12162-CH|
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG12162-CM|
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG12162-CY|
|人 YKL-39/CHI3L2 基因全長cDNA ORF(克隆載體)||HG12162-G|
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG12162-NF|
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG12162-NH|
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG12162-NM|
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG12162-NY|
|人 YKL-39/CHI3L2 基因全長cDNA ORF克隆 (表達載體)||HG12162-UT|
Chondrocyte protein 39 (YKL-39), also known as Chitinase 3-like 2 (CHI3L2), is a secretory protein of articular chondrocytes belonging to the glycosyl hydrolase 18 family. It highest expression is in chondrocytes, followed by synoviocytes, lung and heart. YKL-39/CHI3L2 is not detected in spleen, pancreas, and liver. YKL-39/CHI3L2 may also be expressed in developing brain and placenta. YKL-39/CHI3L2, a cartilage-related protein, is found to induce arthritis accompanied by pathologic changes in bone and cartilage. A better understanding of the immune response against cartilage-related components including YKL-39 may help to elucidate the pathological processes of arthritic disorders. Up regulation of YKL-39/CHI3L2 in osteoarthritic cartilage suggests that YKL-39/CHI3L2 may be a more accurate marker of chondrocyte activation than YKL-40, although it has yet to be established as a suitable marker in synovial fluid and serum. The decreased expression of YKL-40 by osteoarthritic chondrocytes is surprising as increased levels have been reported in rheumatoid and osteoarthritic synovial fluid, where it may derive from activated synovial cells or osteophytic tissue or by increased matrix destruction in the osteoarthritic joint. YKL-39 and YKL-40 are potentially interesting marker molecules for arthritic joint disease because they are abundantly expressed by both normal and osteoarthritic chondrocytes.