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人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤

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Human CDK1 cDNA Clone產品資訊
註冊碼:NM_001786.4
cDNA基因長度:894bp
cDNA基因描述:Full length Clone DNA of Homo sapiens cell division cycle 2, G1 to S and G2 to M transcript variant 1 with N terminal His tag.
基因別名:CDK1, CDC28A, MGC111195, DKFZp686L20222, CDC2
分子種屬:Human
載體:pCMV3-N-His
Plasmid:
限制性酶切位點:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤 on other vectors
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10739-ACGNT$8150
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10739-ACRNT$8150
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG10739-ANGNT$8150
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG10739-ANRNT$8150
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10739-CFNT$7060
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10739-CHNT$7060
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10739-CMNT$7060
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10739-CYNT$7060
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF(克隆載體)HG10739-MNT$2720
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10739-M-FNT$7060
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體)HG10739-M-NNT$7060
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10739-NFNT$7060
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10739-NHNT$7060
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10739-NMNT$7060
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10739-NYNT$7060
人 CDC2 Kinase / CDK1 transcript variant 1 基因全長cDNA ORF克隆 (表達載體)HG10739-UTNT$7060
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研究背景

CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.

參考資料
  • Lee MG, et al. (1987) Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature. 327(6117):31-5.
  • Enserink JM, et al. (2010) An overview of Cdk1-controlled targets and processes. Cell Division. 5(11): 1-41.
  • Ninomiya-Tsuji J, et al. (1991) Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation. Proc Natl Acad Sci. 88(20):9006-10.
  • Zhan Q, et al. (1999) Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45. Oncogene. 18(18):2892-900.
  • Jin S, et al. (2000) The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression. J Biol Chem. 275(22):16602-8.
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