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人 CD48 / SLAMF2 ELISA Pair Set

  • Human CD48/Blast-1 ELISA Pair Set Standard Curve 7134
  • Human CD48 / SLAMF2 ELISA Pair Set
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 CD48 組成成分
Capture Ab:0.2 mg/mL of mouse anti-Human CD48 / SLAMF2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 10797-MM02)
檢測抗體:0.2 mg/mL of rabbit anti-Human CD48 / SLAMF2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.2 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10797-R007)
標準品:Each vial contains 34 ng of recombinant Human CD48 / SLAMF2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 600 pg/mL is recommended.
 CD48 檢測靈敏度
The minimum detectable dose of Human CD48 / SLAMF2 was determined to be approximately 9.38 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
 CD48 產品介紹
The Human CD48 / SLAMF2 ELISA Pair Set is for the quantitative determination of Human CD48 / SLAMF2.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human CD48 / SLAMF2 coated on a 96-well plate. Standards and samples are added to the wells, and any Human CD48 / SLAMF2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human CD48 / SLAMF2 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human CD48 / SLAMF2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
 CD48 儲存方法
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
 CD48 ELISA Pair Set Standard Curve
CD48/Blast-1 研究背景

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

 CD48/Blast-1 參考資料
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
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    貨號: SEKA10797-5
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