CD48 ELISA Kit, Human

Cat: KIT10797
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CD48 ELISA Kit, Human General Information
Product name
CD48 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
2.1 pg/mL
Assay range
9.38-600 pg/mL
Specificity
Recognizes both recombinant and natural Human CD48
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 147.00 261.00 501.00
SD 4.59 5.61 21.54
CV(%) 3.1% 2.1% 4.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 151.00 256.00 487.00
SD 11.41 17.55 46.85
CV(%) 7.5% 6.8% 9.6%
Recovery
The recovery of Human CD48 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 107 99 -115%
supernatant (n=3) 103 99 -107%
Linearity
Serum
  Recovery of detected
1:1 94%
1:2 100%
1:4 106%
1:8 112%
Supernatant
  Recovery of detected
1:1 80%
1:2 94%
1:4 106%
1:8 118%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CD48 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD48 protein in Serum . It contains recombinant Human CD48, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
CD48 ELISA Kit, Human Images
Human CD48/Blast-1 ELISA Kit Standard Curve 73
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human CD48/Blast-1 ELISA Kit Specificity 243
This assay recognizes both recombinant and natural Human CD48. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross -reactivity. No cross-reactivity was observed.
CD48 ELISA Kit, Human Alternative Names
BCM1 ELISA Kit, Human;BLAST ELISA Kit, Human;Blast-1 ELISA Kit, Human;BLAST1 ELISA Kit, Human;hCD48 ELISA Kit, Human;mCD48 ELISA Kit, Human;MEM-102 ELISA Kit, Human;SLAMF2 ELISA Kit, Human
CD48 Background Information

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD48 molecule
References
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    CD48 ELISA Kit, Mouse Lysate KIT50415
    CD48 Matched ELISA Antibody Pair Set,Mouse 46.875 pg/ml SEK50415
    CD48 Matched ELISA Antibody Pair Set,Human 9.38 pg/ml SEKA10797
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