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人 CD40 / TNFRSF5 ELISA Kit

  • Human CD40 / TNFRSF5 ELISA Kit, TYPICAL DATA
  • Human CD40 / TNFRSF5 ELISA Kit, SPECIFICITY
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檢測原理
Solid Phase Sandwich ELISA
預期用途
For the quantitative determination of Human CD40 / TNFRSF5 concentration in urine.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
線性範圍
7.8-500 pg/mL
靈敏度
The minimum detectable dose of Human CD40 / TNFRSF5 is typically less than 2.55 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
試劑盒組分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
儲存方法
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
 CD40 ELISA Kit Standard Curve
Human CD40 / TNFRSF5 ELISA Kit, TYPICAL DATA
[點擊查看大圖]
Caption:
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
 CD40 ELISA Kit Specificity
Human CD40 / TNFRSF5 ELISA Kit, SPECIFICITY
[點擊查看大圖]
Caption:
This assay recognizes both recombinant and natural Human CD40. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD40/TNFRSF5 研究背景

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-stimulatory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

 CD40/TNFRSF5 參考資料
  • van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.
  • Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.
  • Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.
  • Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.
  • Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.
  • Size / Price
    貨號: KIT10774-1
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”