CD40 ELISA Kit, Human General Information
Product name
CD40 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Assay range
7.8-500 pg/mL
Specificity
Recognizes both recombinant and natural Human CD40
Precision
| |
Intra-assay Precision |
| Sample |
1 |
2 |
3 |
| N |
20 |
20 |
20 |
| Mean(pg/mL) |
98.00 |
219.00 |
445.00 |
| SD |
3.62 |
5.05 |
7.35 |
| CV(%) |
3.7% |
2.3% |
1.6% |
| |
Inter-assay Precision |
| Sample |
1 |
2 |
3 |
| N |
20 |
20 |
20 |
| Mean(pg/mL) |
102.00 |
214.00 |
418.00 |
| SD |
7.85 |
17.74 |
55.03 |
| CV(%) |
7.7% |
8.3% |
13.2% |
Recovery
The recovery of Human CD40 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
Average % Recovery |
Range |
| urine (n=3) |
100 |
99 -101% |
Linearity
Urine
| |
Recovery of detected |
| 1:2 |
80% |
| 1:4 |
98% |
| 1:8 |
102% |
| 1:16 |
102% |
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CD40 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40 protein in Urine . It contains recombinant Human CD40, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
CD40 ELISA Kit, Human Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD40. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD40 ELISA Kit, Human Alternative Names
Bp50 ELISA Kit, Human;CDW40 ELISA Kit, Human;p50 ELISA Kit, Human;TNFRSF5 ELISA Kit, Human
CD40 Background Information
CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Immune Checkpoint
Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: ICC Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets Co-stimulatory Immune Checkpoint Targets
Immunotherapy Cancer Immunotherapy Targeted Therapy
Full Name
CD40 molecule, TNF receptor superfamily member 5
References
van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.
