CD40 ELISA Kit, Human

Cat: KIT10774
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CD40 ELISA Kit, Human General Information
Product name
CD40 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Urine
Sensitivity
2.55 pg/mL
Assay range
7.8-500 pg/mL
Specificity
Recognizes both recombinant and natural Human CD40
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 98.00 219.00 445.00
SD 3.62 5.05 7.35
CV(%) 3.7% 2.3% 1.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 102.00 214.00 418.00
SD 7.85 17.74 55.03
CV(%) 7.7% 8.3% 13.2%
Recovery
The recovery of Human CD40 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
urine (n=3) 100 99 -101%
Linearity
Urine
  Recovery of detected
1:2 80%
1:4 98%
1:8 102%
1:16 102%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CD40 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40 protein in Urine . It contains recombinant Human CD40, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
CD40 ELISA Kit, Human Images
Human CD40/TNFRSF5 ELISA Kit Standard Curve 71
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human CD40/TNFRSF5 ELISA Kit Specificity 244
This assay recognizes both recombinant and natural Human CD40. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD40 ELISA Kit, Human Alternative Names
Bp50 ELISA Kit, Human;CDW40 ELISA Kit, Human;p50 ELISA Kit, Human;TNFRSF5 ELISA Kit, Human
CD40 Background Information

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-stimulatory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD40 molecule, TNF receptor superfamily member 5
References
  • van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.
  • Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.
  • Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.
  • Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.
  • Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    CD40 Matched ELISA Antibody Pair Set,Human 3.9 pg/ml SEK10774
    info info
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