|Agonist Activity (Func/A)||Agonist Activity – Human CD28 antibody induce IL2 secretion of the CD4+ T cells. The activating titer (EC50) is typically 0.150.6 μg/mL in the presence of 2 μg/mL Human CD3 (humanized OKT3) antibody(10977-H001) coated on the cell culture plate.|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Targets Co-stimulatory Immune Checkpoint Targets
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-CD28 Antibody (FITC)||Rabbit||Monoclonal||FCM||11524-R007-F|
|Anti-CD28 Antibody (PerCP)||Rabbit||Monoclonal||FCM||11524-R007-C|