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人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤

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Human ENPEP cDNA Clone產品資訊
註冊碼:NM_001977.2
cDNA基因長度:2874bp
cDNA基因描述:Full length Clone DNA of Homo sapiens glutamyl aminopeptidase (aminopeptidase A) with N terminal Flag tag.
基因別名:APA, CD249, gp160
分子種屬:Human
載體:pCMV3-SP-N-FLAG
Plasmid:
限制性酶切位點:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤 on other vectors
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10554-ACGNT$11773
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10554-ACRNT$11773
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10554-CFNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10554-CHNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10554-CMNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10554-CYNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF(克隆載體)HG10554-MNT$2717
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10554-M-FNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體)HG10554-M-NNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10554-NFNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10554-NHNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10554-NMNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10554-NYNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體)HG10554-UTNT$10686
人 ENPEP / Aminopeptidase A 基因全長cDNA ORF克隆 (表達載體)HG10554-UTNT$10686
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研究背景

ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.

參考資料
  • Speth RC, et al. (2008) The significance of brain aminopeptidases in the regulation of the actions of angiotensin peptides in the brain. Heart Fail Rev. 13(3):299-309.
  • Rose JE, et al. (2010) Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Mol Med. 16(7-8):247-53.
  • Pérez I, et al. (2009) Increased APN/CD13 and acid aminopeptidase activities in head and neck squamous cell carcinoma. Head Neck. 31(10):1335-40.
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    貨號: HG10554-NF
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