|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10164-ACG|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10164-ACR|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10164-CF|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10164-CH|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10164-CM|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10164-CY|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF(克隆載體)||HG10164-M|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10164-NF|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10164-NH|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10164-NM|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10164-NY|
|人 IGF1R/CD221/IGF-I R 基因全長cDNA ORF克隆 (表達載體)||HG10164-UT|
The insulin-like growth factor-1 receptor (IGF1R) is a transmembrane tyrosine kinase involved in several biological processes including cell proliferation, differentiation, DNA repair, and cell survival. This a disulfide-linked heterotetrameric transmembrane protein consisting of two α and two β subunits, and among which, the α subunit is extracellular while the β subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF1R signalling pathway is activated in the mammalian nervous system from early developmental stages. Its major effect on developing neural cells is to promote their growth and survival. This pathway can integrate its action with signalling pathways of growth and morphogenetic factors that induce cell fate specification and selective expansion of specified neural cell subsets. Modulation of cell migration is another possible role that IGF1R activation may play in neurogenesis. In the mature brain, IGF-I binding sites have been found in different regions of the brain, and multiple reports confirmed a strong neuroprotective action of the IGF-IR against different pro-apoptotic insults. IGF1R is an important signaling molecule in cancer cells and plays an essential role in the establishment and maintenance of the transformed phenotype. Inhibition of IGF1R signaling thus appears to be a promising strategy to interfere with the growth and survival of cancer cells. IGF1R is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target.