( We provide with CCL26 qPCR primers for gene expression analysis, HP100584 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10577-ACG|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10577-ACR|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10577-CF|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10577-CH|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10577-CM|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10577-CY|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF(克隆載體)||HG10577-M|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10577-M-F|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10577-NF|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10577-NH|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10577-NM|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10577-NY|
|人 CCL26/Eotaxin-3 基因全長cDNA ORF克隆 (表達載體)||HG10577-UT|
The eotaxin subfamily of CC chemokines consists of eotaxin-1/CCL11, eotaxin-2/CCL24 and eotaxin-3/CCL26. All eotaxins induce the trafficking of eosinophils to the sites of inflammation via CC chemokine receptor 3 (CCR3), which is also expressed by several different cell types, including basophils, dendritic cells, smooth muscle cells, epithelial cells and fibroblasts. The sequence similarity between the three eotaxins is limited (<40%), but their functional properties are very similar. Eotaxin-1 and -2 are expressed by both haematopoietic and non-haematopoietic cells, but eotaxin-3 expression has been reported to be limited to non-haematopoietic cells. Interleukin (IL)-4 is the main inducer for eotaxin-3 expression, whereas eotaxin-1 is up-regulated by IL-4 and the proinflammatory cytokine tumour necrosis factor (TNF)-α. Eotaxin-3 is expressed in vascular endothelial cells and human dermal fibroblasts after IL-4 and IL-13 stimulation, and this is dependent upon the IL-4-/IL-13-specific transcription factor, signal transducers and activator of transcription (STAT)-6. Eotaxin-3 is expressed on the surface of IL-4-stimulated endothelial cells and promotes eosinophil transmigration.