Carbonic Anhydrase XIV cDNA ORF Clone, Human, C-His tag

Cat: HG10458-CH
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Carbonic Anhydrase XIV cDNA ORF Clone, Human, C-His tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
960 bp
Description
Full length Clone DNA of Human carbonic anhydrase XIV with C terminal His tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-His
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Carbonic Anhydrase XIV cDNA ORF Clone, Human, C-His tag Alternative Names
CA14 cDNA ORF Clone, Human;CAXiV cDNA ORF Clone, Human
Carbonic Anhydrase XIV Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.

Full Name
carbonic anhydrase XIV
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Parkkila S, et al. (2001) Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. PNAS. 98(4): 1918-23.
  • Carbonic Anhydrase XIV cDNA ORF Clone, Mouse, Related Products

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    Carbonic Anhydrase XIV cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    Carbonic Anhydrase XIV cDNA ORF Clone in Cloning Vector, Human HG10458-U
    Carbonic Anhydrase XIV cDNA ORF Clone, Human, untagged HG10458-UT
    Carbonic Anhydrase XIV Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10458-ACGLN
    Carbonic Anhydrase XIV cDNA ORF Clone, Human, C-GFPSpark® tag HG10458-ACG
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    Carbonic Anhydrase XIV cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10458-CF
    Carbonic Anhydrase XIV cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10458-NF
    Carbonic Anhydrase XIV cDNA ORF Clone, Human, N-His tag HG10458-NH
    Carbonic Anhydrase XIV cDNA ORF Clone, Human, C-HA tag HG10458-CY
    Carbonic Anhydrase XIV cDNA ORF Clone, Human, N-HA tag HG10458-NY
    Carbonic Anhydrase XIV cDNA ORF Clone, Human, C-Myc tag HG10458-CM
    Carbonic Anhydrase XIV cDNA ORF Clone, Human, N-Myc tag HG10458-NM
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