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人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), C-His 標籤

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Human CA13 cDNA Clone產品資訊
註冊碼:NM_198584.2
cDNA基因長度:789bp
cDNA基因描述:Full length Clone DNA of Homo sapiens carbonic anhydrase XIII with C terminal His tag.
基因別名:CAXIII, FLJ37995, MGC59868, CA13
分子種屬:Human
載體:pCMV3-C-His
Plasmid:
限制性酶切位點:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), C-His 標籤 on other vectors
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10461-ACGNT$8150
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10461-ACRNT$8150
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG10461-ANGNT$8150
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG10461-ANRNT$8150
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10461-CFNT$7060
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10461-CHNT$7060
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10461-CMNT$7060
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10461-CYNT$7060
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF(克隆載體)HG10461-MNT$2720
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10461-M-FNT$7060
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10461-NFNT$7060
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10461-NHNT$7060
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10461-NMNT$7060
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10461-NYNT$7060
人 Carbonic Anhydrase XIII/CA13 基因全長cDNA ORF克隆 (表達載體)HG10461-UTNT$7060
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研究背景

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.

參考資料
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
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    貨號: HG10461-CH
    目錄價: 
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    庫存2-3 weeks
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