|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human C2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10154-ACG|
|Human C2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10154-ACR|
|Human C2 ORF mammalian expression plasmid, C-Flag tag||HG10154-CF|
|Human C2 ORF mammalian expression plasmid, C-His tag||HG10154-CH|
|Human C2 ORF mammalian expression plasmid, C-Myc tag||HG10154-CM|
|Human C2 ORF mammalian expression plasmid, C-HA tag||HG10154-CY|
|Human C2 ORF mammalian expression plasmid, Flag tag||HG10154-M-F|
|Human C2 ORF mammalian expression plasmid, N-Flag tag||HG10154-NF|
|Human C2 ORF mammalian expression plasmid, N-His tag||HG10154-NH|
|Human C2 ORF mammalian expression plasmid, N-Myc tag||HG10154-NM|
|Human C2 ORF mammalian expression plasmid, N-HA tag||HG10154-NY|
|Human C2 natural ORF mammalian expression plasmid||HG10154-UT|
Complement component C2 is part of the classical complement pathway which plays a major role in innate immunity against infection. C2 is a glycoprotein synthesized in liver hepatocytes and several other cell types in extrahepatic tissues. This pathway is triggered by a multimolecular complex C1, and subsequently the single-chain form of C2 is cleaved into two chains referred to C2a and C2b by activated C1. The second component of complement (C2) is a multi-domain serine protease that provides catalytic activity for the C3 and C5 convertases of the classical and lectin pathways of human complement. C4b and C2 was investigated by surface plasmon resonance. C2a containing a serine protease domain combines with complement component C4b to form the C3 convertase C4b2a which is responsible for C3 activation, and leads to the stimulation of adaptive immune responses via Lectin pathway. C2 bound to C4b is cleaved by classical (C1s) or lectin (MASP2) proteases to produce C4bC2a. C2 has the same serine protease domain as C4bC2a but in an inactive zymogen-like conformation, requiring cofactor-induced conformational change for activity. Deficiency of C2 (C2D) is the most common genetic deficiency of the complement system, and two types of C2D have been recognized in the context of specific MHC haplotypes. C2D in human is reported to increase susceptibility to infection, and is associated with certain autoimmune diseases, such as rheumatological disorders.