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人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤

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Human BTK cDNA Clone產品資訊
註冊碼:NM_000061.2
cDNA基因長度:1980bp
cDNA基因描述:Full length Clone DNA of Homo sapiens Bruton agammaglobulinemia tyrosine kinase with C terminal Myc tag.
基因別名:AT, ATK, BPK, XLA, IMD1, AGMX1, PSCTK1, MGC126261, MGC126262
分子種屬:Human
載體:pCMV3-C-Myc
Plasmid:pCMV3-BTK-Myc
限制性酶切位點:KpnI + NotI (6kb + 2.03kb)
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1899C/T not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human BTK Gene Plasmid Map
Human Bruton Tyrosine Kinase / BTK Kinase ORF mammalian expression plasmid, C-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤 on other vectors
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10578-ACGNT$8880
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10578-ACRNT$8880
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG10578-ANGNT$8880
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG10578-ANRNT$8880
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10578-CFNT$7790
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10578-CHNT$7790
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10578-CMNT$7790
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10578-CYNT$7790
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF(克隆載體)HG10578-MNT$2720
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10578-NFNT$7790
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10578-NHNT$7790
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10578-NMNT$7790
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10578-NYNT$7790
人 Bruton Tyrosine Kinase / BTK Kinase 基因全長cDNA ORF克隆 (表達載體)HG10578-UTNT$7790
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研究背景

Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.

參考資料
  • Hashimoto S, et al. (1996) Identification of Bruton's tyrosine kinase (Btk) gene mutations and characterization of the derived proteins in 35 X-linked agammaglobulinemia families: a nationwide study of Btk deficiency in Japan. Blood. 88(2): 561-73.
  • Ohta Y, et al. (1994) Genomic organization and structure of Bruton agammaglobulinemia tyrosine kinase: localization of mutations associated with varied clinical presentations and course in X chromosome-linked agammaglobulinemia. PNAS. 91(19): 9062-6.
  • Smith C, et al. (1994) Expression of Bruton's agammaglobulinemia tyrosine kinase gene, BTK, is selectively down-regulated in T lymphocytes and plasma cells. The Journal of Immunology. 152(2): 557-65.
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