BMP-2 ELISA Kit, Human

Cat: KIT10426
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BMP-2 ELISA Kit, Human General Information
Product name
BMP-2 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Recombinant protein
Sensitivity
13.9 pg/mL
Assay range
23.44-1500 pg/mL
Specificity
Recognizes both recombinant and natural Human BMP-2
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 258.00 518.00 971.00
SD 7.58 11.74 30.18
CV(%) 3.0% 2.3% 3.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 216.00 461.00 852.00
SD 23.62 35.25 88.90
CV(%) 11.0% 7.6% 10.4%
Recovery
The recovery of Human BMP2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
supernatant (n=3) 78 76 -80%
Linearity
Supernatant
  Recovery of detected
1:2 99%
1:4 104%
1:8 96%
1:16 81%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This BMP-2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP-2 protein in Recombinant protein . It contains recombinant Human BMP-2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
BMP-2 ELISA Kit, Human Images
Human BMP-2 ELISA Kit Standard Curve 64
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human BMP-2 ELISA Kit Specificity 153
This assay recognizes recombinant Human NOV. The factors listed above were prepared at 50 or 100 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
BMP-2 ELISA Kit, Human Alternative Names
BDA2 ELISA Kit, Human;BDA2A ELISA Kit, Human;BMP-2 ELISA Kit, Human;BMP2A ELISA Kit, Human
BMP-2 Background Information

BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.

Full Name
bone morphogenetic protein 2
References
  • Jiao X, et al. (2007) Heparan sulfate proteoglycans (HSPGs) modulate BMP-2 osteogenic bioactivity in C2C12 cells. J Biol Chem. 282(2):1080-6.
  • Michon F, et al. (2008) BMP-2 and BMP-7 play antagonistic roles in feather induction. Development 135 (16):2797-805.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    BMP-2 Matched ELISA Antibody Pair Set,Human 31.25 pg/ml SEK10426
    info info
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