( We provide with B4GALT1 qPCR primers for gene expression analysis, HP101121 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG11220-ACG|
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG11220-ACR|
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG11220-CF|
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG11220-CH|
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG11220-CM|
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG11220-CY|
|人 B4GALT1/GGTB2 基因全長cDNA ORF(克隆載體)||HG11220-M|
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG11220-NF|
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG11220-NH|
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG11220-NM|
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG11220-NY|
|人 B4GALT1/GGTB2 基因全長cDNA ORF克隆 (表達載體)||HG11220-UT|
Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.