|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG11221-ACG|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG11221-ACR|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG11221-CF|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG11221-CH|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG11221-CM|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG11221-CY|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF(克隆載體)||HG11221-M|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體)||HG11221-M-N|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG11221-NF|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG11221-NH|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG11221-NM|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG11221-NY|
|人 Apolipoprotein H/APOH 基因全長cDNA ORF克隆 (表達載體)||HG11221-UT|
Apolipoprotein H (APOH), also known as Beta-2-glycoprotein 1, Activated protein C-binding protein, B2GPI, and B2G1, is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. It is an essential cofactor for the binding of certain antiphospholipid antibodies (APA) to anionic phospholipid. APOH binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. APOH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. APOH appears to completely inhibit serotonin release by the platelets and prevents subsequent waves of the ADP-induced aggregation. The activity of APOH appears to involve the binding of agglutenating, negatively charged compounds, and inhibits agglutenation by the contact activation of the intrinsic blood coagulation pathway. APOH causes a reduction of the prothrombinase binding sites on platelets and reduces the activation caused by collagen when thrombin is present at physiological serum concentrations of APOH suggesting a regulatory role of APOH in coagulation. APOH plasma concentrations are strongly associated to metabolic syndrome alterations and vascular disease in type 2 diabetic and could be considered as a clinical marker of cardiovascular risk. APOH is found on several classes of lipoproteins, and is involved in the activation of lipoprotein lipase in lipid metabolism. This single-chain glycoprotein also has been implicated in several physiologic pathways including coagulation and the production of hypertension, which are related to the pathogenesis of primary cerebral hemorrhage (PICH).