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人 AMPK (G1/B2/A1) Heterotrimer 杆狀病毒-昆蟲 過表達細胞裂解液 (陽性對照)

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Human AMPK Cells Transfected Lysate Product Information
表達宿主:Baculovirus-Insect cells
點擊查看詳細資訊:Baculovirus-Insect Cell lysate that Human AMPK (G1/B2/A1) Heterotrimer transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Sequence information:A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
預測N端:Met & Met & His
分子量:The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
分子種屬:Human
Human AMPK Transfected / Overexpression Cell Lysate Usage Guide
製備方法:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解緩衝液:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
質控:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
穩定性:Samples are stable for up to twelve months from date of receipt.
推薦使用方法:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
儲存緩衝液:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
儲存方法:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
應用小提示:Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
Size / Price
貨號: CT005-H0907BL-300
目錄價: 
單價:      (You Save: )
規格:
300 µg
數量:+-
庫存2 weeks
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