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人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤

產品資料評論相關產品實驗方法
Human ACVR1B cDNA Clone產品資訊
註冊碼:NM_004302.3
cDNA基因長度:1518bp
cDNA基因描述:Full length Clone DNA of Homo sapiens activin A receptor, type I B, transcript variant 1 with C terminal Myc tag.
基因別名:ALK4, SKR2, ACTRIB, ACVRLK4
分子種屬:Human
載體:pCMV3-C-Myc
Plasmid:
限制性酶切位點:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤 on other vectors
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10583-ACGNT$8880
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10583-ACRNT$8880
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10583-CFNT$7790
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10583-CHNT$7790
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10583-CMNT$7790
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10583-CYNT$7790
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF(克隆載體)HG10583-MNT$2720
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10583-M-FNT$7790
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體)HG10583-M-NNT$7790
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10583-NFNT$7790
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10583-NHNT$7790
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10583-NMNT$7790
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10583-NYNT$7790
人 ALK-4 / ACVR1B transcript variant 1 基因全長cDNA ORF克隆 (表達載體)HG10583-UTNT$7790
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研究背景

ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination.

參考資料
  • Chen Y, et al. (2005) Developmental analysis of activin-like kinase receptor-4 (ALK4) expression in Xenopus laevis. 232(2): 393-8.
  • J. Massagu. (1998) TGF- SIGNAL TRANSDUCTION. Annual Review of Biochemistry. 67: 753-91.
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