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人 AFP / alpha-fetoprotein ELISA Kit

  • Human AFP / alpha-fetoprotein ELISA Kit, TYPICAL DATA
  • Human AFP / alpha-fetoprotein ELISA Kit, SPECIFICITY
產品資料評論相關產品
檢測原理
Solid Phase Sandwich ELISA
預期用途
For the quantitative determination of Human AFP / alpha-fetoprotein concentration in serum.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
線性範圍
15.63-1000 pg/mL
靈敏度
The minimum detectable dose of Human AFP / alpha-fetoprotein is typically less than 5.59 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
試劑盒組分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
儲存方法
 AFP ELISA Kit Standard Curve
Human AFP / alpha-fetoprotein ELISA Kit, TYPICAL DATA
[點擊查看大圖]
Caption:
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
 AFP ELISA Kit Specificity
Human AFP / alpha-fetoprotein ELISA Kit, SPECIFICITY
[點擊查看大圖]
Caption:
This assay recognizes both recombinant and natural Human AFP. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of Leptin at 20 ng/mL in a mid-range recombinant human AFP control were assayed for interference. No significant interference was observed.

Coating: Coat corresponding Protein (20 μg/mL in pH 9.6 carbonate buffer) in NUNC plate
Sample: Add the indicated amounts of M13 Bacteriophages displaying Rabbit/Mouse Fab targeted for different proteins;
Primary Antibody: M13 Antibody (11973-MM05T), 0.365 μg/mL
Secondary Antibody: anti-mouse F(ab)2 Antibody/HRP
Alpha-fetoprotein/AFP 研究背景

Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

 Alpha-fetoprotein/AFP 參考資料
  • Mizejewski GJ. (2001) Alpha-fetoprotein Structure and Function: Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408.
  • Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
  • Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
  • Size / Price
    貨號: KIT12177-1
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    Datasheet & Documentation

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