Alpha-fetoprotein ELISA Kit, Human

Cat: KIT12177
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Alpha-fetoprotein ELISA Kit, Human General Information
Product name
Alpha-fetoprotein ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
5.59 pg/mL
Assay range
15.63-1000 pg/mL
Specificity
Recognizes both recombinant and natural Human Alpha-fetoprotein
Interference
Preparations of Leptin at 20 ng/mL in a mid-range recombinant human AFP control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 63.95 121.41 271.84
SD 4.67 7.31 13.54
CV(%) 7.3% 6.0% 5.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 105.20 211.97 419.59
SD 13.40 2.83 19.70
CV(%) 12.7% 1.3% 4.7%
Recovery
The recovery of Human AFP spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 90 85-94%
Linearity
Serum
  Recovery of detected
1:2 114%
1:4 104%
1:8 83%
1:16 83%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This Alpha-fetoprotein ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Alpha-fetoprotein protein in Serum . It contains recombinant Human Alpha-fetoprotein, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Alpha-fetoprotein ELISA Kit, Human Images
Human Alpha-fetoprotein/AFP ELISA Kit Standard Curve 114
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human Alpha-fetoprotein/AFP ELISA Kit Specificity 255
This assay recognizes both recombinant and natural Human AFP. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of Leptin at 20 ng/mL in a mid-range recombinant human AFP control were assayed for interference. No significant interference was observed.
Alpha-fetoprotein ELISA Kit, Human Alternative Names
AFPD ELISA Kit, Human;Alpha-fetoprotein ELISA Kit, Human;FETA ELISA Kit, Human;HPAFP ELISA Kit, Human
Alpha-fetoprotein Background Information

Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).

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Full Name
alpha-fetoprotein
References
  • Mizejewski GJ. (2001) Alpha-fetoprotein Structure and Function: Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408.
  • Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
  • Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    Alpha-fetoprotein Matched ELISA Antibody Pair Set,Human 11 pg/ml SEK12177
    info info
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