( We provide with ADAMTSL1 qPCR primers for gene expression analysis, HP102236 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG13550-ACG|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG13550-ACR|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG13550-CF|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG13550-CH|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG13550-CM|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG13550-CY|
|人 ADAMTSL1 基因全長cDNA ORF(克隆載體)||HG13550-G|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體)||HG13550-G-N|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG13550-NF|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG13550-NH|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG13550-NM|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG13550-NY|
|人 ADAMTSL1 基因全長cDNA ORF克隆 (表達載體)||HG13550-UT|
ADAMTSL1 is a secreted molecule resembling members of the ADAMTS protein family of matrix metalloproteinases. Both ADAMTS proteins and ADAM protein family contain a disintegrin and a metalloprotease domain. Metallospondins is collective term for members of ADAMTS protein family. ADAMTS proteins lack the EGF-like domain found normally in members of the ADAM protein family. They also do not possess the canonical disintegrin sequence found in the ADAM protein family. It contains the domains found in members of the ADAMTS protein family with the exception of the pro-metalloprotease and the disintegrin-like domain typical of this family. ADAMTSL1 gene is expressed in adult skeletal muscle. ADAMTSL1 may play an important role in the extracellular matrix as it is deposited in the cell substratum in a punctate fashion and is excluded from focal contacts.