|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG11243-ACG|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG11243-ACR|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG11243-CF|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG11243-CH|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG11243-CM|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG11243-CY|
|人 ACPL2 基因全長cDNA ORF(克隆載體)||HG11243-M|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體)||HG11243-M-N|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG11243-NF|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG11243-NH|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG11243-NM|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG11243-NY|
|人 ACPL2 基因全長cDNA ORF克隆 (表達載體)||HG11243-UT|
acid phosphatase-like protein 2, also known as ACPL2, is a secreted protein which belongs to the histidine acid phosphatase family. A large-scale effort, termed the Secreted Protein Discovery Initiative (SPDI), was undertaken to identify novel secreted and transmembrane proteins. In the first of several approaches, a biological signal sequence trap in yeast cells was utilized to identify cDNA clones encoding putative secreted proteins. A second strategy utilized various algorithms that recognize features such as the hydrophobic properties of signal sequences to identify putative proteins encoded by expressed sequence tags (ESTs) from human cDNA libraries. A third approach surveyed ESTs for protein sequence similarity to a set of known receptors and their ligands with the BLAST algorithm. Finally, both signal-sequence prediction algorithms and BLAST were used to identify single exons of potential genes from within human genomic sequence.